Proteintech, 55462-1-AP, Myosin Light Chain 2/MLC-2V Polyclonal antibody

Size: 20ul / 150ul The Myosin Light Chain 2/MLC-2V (55462-1-AP) by Proteintech is a Polyclonal antibody targeting Myosin Light Chain 2/MLC-2V in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat heart tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissue, human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:20000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, light chain 2, regulatory, cardiac, slow Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 18-20 kDa GenBank Accession Number: NM_000432 Gene Symbol: Myosin Light Chain 2 Gene ID (NCBI): 4633 RRID: AB_2881342 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P10916 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.