Proteintech, 55474-1-AP, GSTM2 Polyclonal antibody

Size: 20ul / 150ul The GSTM2 (55474-1-AP) by Proteintech is a Polyclonal antibody targeting GSTM2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 55474-1-AP targets GSTM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: glutathione S-transferase mu 2 (muscle) Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: NM_000848 Gene Symbol: GSTM2 Gene ID (NCBI): 2946 RRID: AB_3086453 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P28161 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.