Proteintech, 60007-1-Ig, TGFBI / BIGH3 Monoclonal antibody

Size: 20ul / 150ul The TGFBI / BIGH3 (60007-1-Ig) by Proteintech is a Monoclonal antibody targeting TGFBI / BIGH3 in WB, IHC, IF-P, IP, ELISA applications with reactivity to human samples 60007-1-Ig targets TGFBI / BIGH3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human kidney tissue Positive IP detected in: HeLa cells Positive IHC detected in: human colon tissue, human skin cancer tissue, human colon cancer tissue, human placenta tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0241 Product name: Recombinant human BIGH3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 199-406 aa of BC000097 Sequence: NIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSG Predict reactive species Full Name: transforming growth factor, beta-induced, 68kDa Calculated Molecular Weight: 683 aa, 75 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC000097 Gene Symbol: TGFBI Gene ID (NCBI): 7045 ENSEMBL Gene ID: ENSG00000120708 RRID: AB_10896828 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15582 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.