Proteintech, 60058-1-Ig, PLOD3 Monoclonal antibody

Size: 20ul / 150ul The PLOD3 (60058-1-Ig) by Proteintech is a Monoclonal antibody targeting PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 60058-1-Ig targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, human placenta tissue, PC-3 cells, K-562 cells, LNCaP cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Positive IP detected in: HeLa cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1480 Product name: Recombinant human PLOD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 339-738 aa of BC011674 Sequence: HEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP Predict reactive species Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 Calculated Molecular Weight: 738 aa, 85 kDa Observed Molecular Weight: 80-85 kDa GenBank Accession Number: BC011674 Gene Symbol: PLOD3 Gene ID (NCBI): 8985 RRID: AB_2165779 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60568 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.