Proteintech, 60064-1-Ig, HDGF Monoclonal antibody

Size: 20ul / 150ul The HDGF (60064-1-Ig) by Proteintech is a Monoclonal antibody targeting HDGF in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 60064-1-Ig targets HDGF in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SMMC-7721 cells, A549 cells, HeLa cells, HepG2 cells, L02 cells, rat liver tissue Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like) Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC018991 Gene Symbol: HDGF Gene ID (NCBI): 3068 RRID: AB_2117098 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P51858 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.