Proteintech, 60081-1-Ig, Fibulin 5 Monoclonal antibody

Size: 20ul / 150ul The Fibulin 5 (60081-1-Ig) by Proteintech is a Monoclonal antibody targeting Fibulin 5 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 60081-1-Ig targets Fibulin 5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: MCF-7 cells, pig heart tissue, human placenta tissue, human heart tissue, rat heart tissue Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:100 Background Information Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2859 Product name: Recombinant human FBLN5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 186-448 aa of BC022280 Sequence: GSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFSEFLCQHECVNQPGTYFCSCPPGYILLDDNRSCQDINECEHRNHTCNLQQTCYNLQGGFKCIDPIRCEEPYLRISDNRCMCPAENPGCRDQPFTILYRDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPREIQLDLEMITVNTVINFRGSSVIRLRIYVSQYPF Predict reactive species Full Name: fibulin 5 Calculated Molecular Weight: 448 aa, 50 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC022280 Gene Symbol: Fibulin 5 Gene ID (NCBI): 10516 RRID: AB_2231920 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UBX5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.