Proteintech, 60084-1-Ig, DGCR8 Monoclonal antibody

Size: 20ul / 150ul The DGCR8 (60084-1-Ig) by Proteintech is a Monoclonal antibody targeting DGCR8 in WB, IP, ELISA applications with reactivity to human, mouse, rat, pig samples 60084-1-Ig targets DGCR8 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, BxPC-3 cells, RAW 264.7 cells, A431 cells, Jurkat cells, K-562 cells, ROS1728 cells Positive IP detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4871 Product name: Recombinant human DGCR8 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 167-517 aa of BC009323 Sequence: VKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV Predict reactive species Full Name: DiGeorge syndrome critical region gene 8 Calculated Molecular Weight: 773 aa, 86 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC009323 Gene Symbol: DGCR8 Gene ID (NCBI): 54487 RRID: AB_2090985 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8WYQ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.