Proteintech, 60177-1-Ig, CKM-Specific Monoclonal antibody

Size: 20ul / 150ul The CKM-Specific (60177-1-Ig) by Proteintech is a Monoclonal antibody targeting CKM-Specific in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 60177-1-Ig targets CKM-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Human skeletal muscle, human skeletal muscle tissue Positive IHC detected in: human liver tissue, mouse heart tissue, mouse testis tissue, rat brain tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: creatine kinase, muscle Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43 kDa, 90 kDa, 130 kDa GenBank Accession Number: BC007462 Gene Symbol: CKM Gene ID (NCBI): 1158 RRID: AB_10897180 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06732 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.