Proteintech, 60224-1-Ig, CD44 Monoclonal antibody

Size: 20ul / 150ul The CD44 (60224-1-Ig) by Proteintech is a Monoclonal antibody targeting CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to human, pig samples 60224-1-Ig targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, human spleen tissue, HeLa cells, A549 cells, U-251 cells, HUVEC cells, pig spleen tissue Positive IP detected in: HeLa cells Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. Specification Tested Reactivity: human, pig Cited Reactivity: human, rat, pig, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7633 Product name: Recombinant human CD44 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 28-380 aa of BC004372 Sequence: CRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATSTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPREDSHST Predict reactive species Full Name: CD44 molecule (Indian blood group) Calculated Molecular Weight: 742 aa, 82 kDa Observed Molecular Weight: 80-95 kDa GenBank Accession Number: BC004372 Gene Symbol: CD44 Gene ID (NCBI): 960 ENSEMBL Gene ID: ENSG00000026508 RRID: AB_11042767 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P16070 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.