Proteintech, 60257-1-Ig, Dynamitin (1-401 AA) Monoclonal antibody

Size: 20ul / 150ul The Dynamitin (1-401 AA) (60257-1-Ig) by Proteintech is a Monoclonal antibody targeting Dynamitin (1-401 AA) in WB, IHC, ELISA applications with reactivity to human, mouse samples 60257-1-Ig targets Dynamitin (1-401 AA) in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human brain tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin. Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1902 Product name: Recombinant human dynactin-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-401 aa of BC014083 Sequence: MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK Predict reactive species Full Name: dynactin 2 (p50) Calculated Molecular Weight: 401 aa, 44 kDa Observed Molecular Weight: 52+150 kDa GenBank Accession Number: BC014083 Gene Symbol: Dynamitin Gene ID (NCBI): 10540 RRID: AB_2881378 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13561 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.