Proteintech, 60284-1-Ig, ATP5C1 Monoclonal antibody

Size: 20ul / 150ul The ATP5C1 (60284-1-Ig) by Proteintech is a Monoclonal antibody targeting ATP5C1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, pig, rabbit samples 60284-1-Ig targets ATP5C1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig, rabbit samples. Tested Applications Positive WB detected in: human heart tissue, Caki-1 cells, fetal human brain tissue, HeLa cells, human skeletal muscle tissue, TF-1 cells, Rabbit heart tissue, Pig heart tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057). Specification Tested Reactivity: human, pig, rabbit Cited Reactivity: human, zebrafish Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1343 Product name: Recombinant human ATP5C1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC000931 Sequence: MFSRAGVAGLSAWTLQPQWIQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD Predict reactive species Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC000931 Gene Symbol: ATP5C1 Gene ID (NCBI): 509 RRID: AB_2881402 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P36542 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.