Proteintech, 60317-1-Ig, GOT1 Monoclonal antibody

Size: 20ul / 150ul The GOT1 (60317-1-Ig) by Proteintech is a Monoclonal antibody targeting GOT1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, chicken samples 60317-1-Ig targets GOT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, chicken samples. Tested Applications Positive WB detected in: LNCaP cells, rat brain tissue, rat heart tissue, mouse brain tissue, K-562 cells, chicken brain, chicken heart Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors. Specification Tested Reactivity: human, mouse, rat, chicken Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7012 Product name: Recombinant human GOT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-413 aa of BC000498 Sequence: MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ Predict reactive species Full Name: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 40-46 kDa GenBank Accession Number: BC000498 Gene Symbol: GOT1 Gene ID (NCBI): 2805 RRID: AB_2881428 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P17174 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.