Proteintech, 60337-1-Ig, HEXA Monoclonal antibody

Size: 20ul / 150ul The HEXA (60337-1-Ig) by Proteintech is a Monoclonal antibody targeting HEXA in WB, ELISA applications with reactivity to human, mouse, rat, rabbit samples 60337-1-Ig targets HEXA in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: HeLa cells, rabbit testis tissue, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570). Specification Tested Reactivity: human, mouse, rat, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1854 Product name: Recombinant human HEXA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-529 aa of BC018927 Sequence: MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT Predict reactive species Full Name: hexosaminidase A (alpha polypeptide) Calculated Molecular Weight: 529 aa, 60 kDa Observed Molecular Weight: 50-54 kDa, 60-67 kDa GenBank Accession Number: BC018927 Gene Symbol: HEXA Gene ID (NCBI): 3073 RRID: AB_2881446 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06865 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.