Proteintech, 60348-1-Ig, Melan-A Monoclonal antibody

Size: 20ul / 150ul The Melan-A (60348-1-Ig) by Proteintech is a Monoclonal antibody targeting Melan-A in IHC, ELISA applications with reactivity to human samples 60348-1-Ig targets Melan-A in IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human malignant melanoma tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13387 Product name: Recombinant human MLANA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-118 aa of BC014423 Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP Predict reactive species Full Name: melan-A Calculated Molecular Weight: 13 kDa GenBank Accession Number: BC014423 Gene Symbol: MelanA Gene ID (NCBI): 2315 RRID: AB_2881457 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16655 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.