Proteintech, 60597-1-Ig, Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody

Size: 20ul / 100ul The Phospho-PERK/EIF2AK3 (Ser719) (60597-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications with reactivity to human, mouse samples 60597-1-Ig targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, NIH/3T3 cells, PC-3 cells, Jurkat cells, Calyculin A treated Jurkat cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570) Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 Calculated Molecular Weight: 1116 aa, 125 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC126354 Gene Symbol: PERK Gene ID (NCBI): 9451 RRID: AB_3670269 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9NZJ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.