Proteintech, 60929-1-Ig, ERK1/2 Monoclonal antibody

Size: 20ul / 150ul The ERK1/2 (60929-1-Ig) by Proteintech is a Monoclonal antibody targeting ERK1/2 in WB, ELISA applications with reactivity to human, mouse, rat samples 60929-1-Ig targets ERK1/2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, HUVEC cells, COLO 320 cells, HepG2 cells, MOLT-4 cells, HSC-T6 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information ERK1 and ERK2 belong to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with a molecular mass of 38-44 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 1 Calculated Molecular Weight: 360 aa, 41 kDa Observed Molecular Weight: 38-44 kDa GenBank Accession Number: BC017832 Gene Symbol: ERK2 Gene ID (NCBI): 5594 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P28482 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.