Proteintech, 65080-1-Ig, Anti-Mouse CD16/CD32 (2.4G2)

Size: 100ug / 500ug The CD16/CD32 (65080-1-Ig) by Proteintech is a Monoclonal antibody targeting CD16/CD32 in FC applications with reactivity to mouse samples 65080-1-Ig targets CD16/CD32 in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining. Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rat / IgG2b, kappa Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: Fc receptor, IgG, low affinity IIb GenBank Accession Number: BC038070 Gene Symbol: CD16/32 Gene ID (NCBI): 14130 RRID: AB_2918383 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P08101 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.