Proteintech, 65099-1-Ig, Anti-Human CD28 (CD28.2)

Size: 100ug / 500ug The CD28 (65099-1-Ig) by Proteintech is a Monoclonal antibody targeting CD28 in FC applications with reactivity to Human samples 65099-1-Ig targets CD28 in FC, IHC, Cell treatment applications and shows reactivity with Human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520). Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: CD28 molecule Calculated Molecular Weight: 220 aa, 25 kDa GenBank Accession Number: BC093698 Gene Symbol: CD28 Gene ID (NCBI): 940 ENSEMBL Gene ID: ENSG00000178562 RRID: AB_2918401 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P10747 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.