Proteintech, 65117-1-Ig, Anti-Mouse CD44 (IM7)

Size: 500ug The CD44 (65117-1-Ig) by Proteintech is a Monoclonal antibody targeting CD44 in IF/ICC, FC applications with reactivity to Human, Mouse samples 65117-1-Ig targets CD44 in IHC, IF/ICC, FC applications and shows reactivity with Human, Mouse samples. Tested Applications Positive IF/ICC detected in: HeLa cells Positive FC detected in: mouse splenocytes Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:400 Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Specification Tested Reactivity: Human, Mouse Host / Isotype: Rat / IgG2b, kappa Class: Monoclonal Type: Antibody Immunogen: Dexamethasone-induced myeloid leukemia M1 cells Predict reactive species Full Name: CD44 antigen GenBank Accession Number: BC051388 Gene Symbol: Cd44 Gene ID (NCBI): 12505 RRID: AB_2918413 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15379 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.