Proteintech, 65129-1-Ig, Anti-Mouse CD40L/CD154 (MR1)

Size: 100ug / 500ug The CD40L/CD154 (65129-1-Ig) by Proteintech is a Monoclonal antibody targeting CD40L/CD154 in FC applications with reactivity to mouse samples 65129-1-Ig targets CD40L/CD154 in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: PMA and ionomycin stimulated mouse CD4+ T cells Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1. Specification Tested Reactivity: mouse Host / Isotype: Armenian Hamster / IgG Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: CD40 ligand GenBank Accession Number: BC119225 Gene Symbol: CD154 Gene ID (NCBI): 21947 RRID: AB_3670275 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P27548 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.