Proteintech, 65140-1-Ig, Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

Size: 500ug The Ly-6G/Ly-6C (Gr-1) (65140-1-Ig) by Proteintech is a Monoclonal antibody targeting Ly-6G/Ly-6C (Gr-1) in FC applications with reactivity to mouse samples 65140-1-Ig targets Ly-6G/Ly-6C (Gr-1) in WB, FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse bone marrow cells Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469). Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rat / IgG2b, kappa Class: Monoclonal Type: Antibody Immunogen: Raised against granulocytes of mouse origin Predict reactive species Full Name: lymphocyte antigen 6 complex, locus G GenBank Accession Number: X70920 Gene Symbol: Ly-6G Gene ID (NCBI): 546644 RRID: AB_2881475 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P35461 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.