Proteintech, 65150-1-Ig, Anti-Human CD45RO (UCHL1)

Size: 500ug The CD45RO (65150-1-Ig) by Proteintech is a Monoclonal antibody targeting CD45RO in IHC, IF-P, FC applications with reactivity to human samples 65150-1-Ig targets CD45RO in IHC, IF-P, FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human tonsillitis tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Positive FC detected in: human PBMCs Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: IL-2 dependent T cell line, CA1 Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, C GenBank Accession Number: BC014239 Gene Symbol: CD45 Gene ID (NCBI): 5788 ENSEMBL Gene ID: ENSG00000081237 RRID: AB_2881476 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08575 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.