Proteintech, 65161-1-Ig, Anti-Human MICA/MICB (6D4)

Size: 500ug The MICA/MICB (65161-1-Ig) by Proteintech is a Monoclonal antibody targeting MICA/MICB in FC applications with reactivity to Human samples 65161-1-Ig targets MICA/MICB in WB, FC applications and shows reactivity with Human samples. Tested Applications Positive FC detected in: HeLa cells Recommended dilution Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB. Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Mouse / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: Transfected C1R cells expressing MICA Predict reactive species Full Name: MHC class I polypeptide-related sequence A Calculated Molecular Weight: 383 aa, 43 kDa GenBank Accession Number: BC016929 Gene Symbol: MICA Gene ID (NCBI): 100507436 RRID: AB_2881478 Conjugate: Unconjugated Form: Liquid Purification Method: Purified by protein-A affinity chromatography UNIPROT ID: Q29983 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.