Proteintech, 65224-1-Ig, Anti-Rat CD86 (24F)

Size: 100ug / 500ug The CD86 (65224-1-Ig) by Proteintech is a Monoclonal antibody targeting CD86 in FC applications with reactivity to rat samples 65224-1-Ig targets CD86 in IF, FC applications and shows reactivity with rat samples. Tested Applications Positive FC detected in: LPS-stimulated Wistar rat splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Specification Tested Reactivity: rat Cited Reactivity: rat Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line Predict reactive species Full Name: CD86 molecule Calculated Molecular Weight: 36 kDa GenBank Accession Number: NM_020081 Gene Symbol: Cd86 Gene ID (NCBI): 56822 RRID: AB_3670278 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: O35531 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.