Proteintech, 65286-1-Ig, Anti-Human IL-10 (7B8G3)

Size: 100ug The IL-10 (65286-1-Ig) by Proteintech is a Monoclonal antibody targeting IL-10 in FC (Intra) applications with reactivity to human samples 65286-1-Ig targets IL-10 in FC (Intra) applications and shows reactivity with human samples. Tested Applications Positive FC (Intra) detected in: LPS and Brefeldin A treated human PBMCs Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension Background Information Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Product name: HumanKine® recombinant human IL-10 protein Source: -derived, Tag: Domain: of Sequence: Predict reactive species Full Name: interleukin 10 Calculated Molecular Weight: 21 kDa GenBank Accession Number: BC104252 Gene Symbol: IL-10 Gene ID (NCBI): 3586 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P22301 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.