Proteintech, 65292-1-Ig, O-GlcNAc Monoclonal antibody

Size: 20ul / 150ul The O-GlcNAc (65292-1-Ig) by Proteintech is a Monoclonal antibody targeting O-GlcNAc in WB applications with reactivity to n/a samples 65292-1-Ig targets O-GlcNAc in WB applications and shows reactivity with n/a samples. Tested Applications Positive WB detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-20000 Background Information O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769). Specification Tested Reactivity: n/a Host / Isotype: Mouse / IgM Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: O-GlcNAc RRID: AB_3670285 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.