Proteintech, 66001-2-Ig, GST Tag Monoclonal antibody

Size: 150ul The GST Tag (66001-2-Ig) by Proteintech is a Monoclonal antibody targeting GST Tag in WB, IP, ELISA applications with reactivity to Recombinant protein, Schistosoma japonicum samples 66001-2-Ig targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples. Tested Applications Positive WB detected in: Recombinant protein Positive IP detected in: E. coli lysate Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins. Specification Tested Reactivity: Recombinant protein, Schistosoma japonicum Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: GST Tag Calculated Molecular Weight: 26 kDa GenBank Accession Number: FN315687 RRID: AB_2881488 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.