Proteintech, 66017-1-Ig, TPPII Monoclonal antibody

Size: 20ul / 150ul The TPPII (66017-1-Ig) by Proteintech is a Monoclonal antibody targeting TPPII in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66017-1-Ig targets TPPII in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Hela cells, Jurkat Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5296 Product name: Recombinant human TPP2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 900-1249 aa of BC039905 Sequence: LERLKDLPFIVSHRLSNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF Predict reactive species Full Name: tripeptidyl peptidase II Calculated Molecular Weight: 1249 aa, 138 kDa Observed Molecular Weight: 135 kDa GenBank Accession Number: BC039905 Gene Symbol: TPPII Gene ID (NCBI): 7174 RRID: AB_11044195 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P29144 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.