Proteintech, 66036-1-Ig, ASS1 Monoclonal antibody

Size: 20ul / 150ul The ASS1 (66036-1-Ig) by Proteintech is a Monoclonal antibody targeting ASS1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig samples 66036-1-Ig targets ASS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: pig liver tissue, HeLa cells, human liver tissue, L02 cells Positive IHC detected in: human liver tissue, human liver cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241). Specification Tested Reactivity: human, pig Cited Reactivity: human, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9314 Product name: Recombinant human ASS1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 149-412 aa of BC009243 Sequence: EFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK Predict reactive species Full Name: argininosuccinate synthetase 1 Calculated Molecular Weight: 412 aa, 47 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC009243 Gene Symbol: ASS1 Gene ID (NCBI): 445 RRID: AB_11042883 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P00966 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.