Proteintech, 66066-1-Ig, CAPZA1 Monoclonal antibody

Size: 20ul / 150ul The CAPZA1 (66066-1-Ig) by Proteintech is a Monoclonal antibody targeting CAPZA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66066-1-Ig targets CAPZA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells, ROS1728 cells, PC-12 cells, RAW 264.7 cells, NIH/3T3 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:800 Background Information CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18841 Product name: Recombinant human CAPZA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-286 aa of BC000144 Sequence: MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA Predict reactive species Full Name: capping protein (actin filament) muscle Z-line, alpha 1 Calculated Molecular Weight: 286 aa, 33 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC000144 Gene Symbol: CAPZA1 Gene ID (NCBI): 829 RRID: AB_11042588 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P52907 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.