Proteintech, 66107-1-Ig, MAOB Monoclonal antibody

Size: 20ul / 150ul The MAOB (66107-1-Ig) by Proteintech is a Monoclonal antibody targeting MAOB in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 66107-1-Ig targets MAOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: fetal human brain tissue, human brain tissue, human kidney tissue, HeLa cells, HepG2 cells, HuH-7 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449). Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17918 Product name: Recombinant human MAOB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-331 aa of BC022494 Sequence: MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDHVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIQSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDT Predict reactive species Full Name: monoamine oxidase B Calculated Molecular Weight: 520 aa, 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC022494 Gene Symbol: MAOB Gene ID (NCBI): 4129 RRID: AB_2881506 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P27338 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.