Proteintech, 66112-1-Ig, Iduronate 2 sulfatase Monoclonal antibody

Size: 20ul / 150ul The Iduronate 2 sulfatase (66112-1-Ig) by Proteintech is a Monoclonal antibody targeting Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66112-1-Ig targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: Human placenta, rat brain tissue, human brain tissue, mouse brain tissue, pig brain tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19095 Product name: Recombinant human IDS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-312 aa of BC006170 Sequence: MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK Predict reactive species Full Name: iduronate 2-sulfatase Calculated Molecular Weight: 312aa,35 kDa; 550aa,62 kDa Observed Molecular Weight: 76 kDa, 55 kDa GenBank Accession Number: BC006170 Gene Symbol: IDS Gene ID (NCBI): 3423 RRID: AB_2881511 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P22304 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.