Proteintech, 66151-1-Ig, HBE1-Specific Monoclonal antibody

Size: 20ul / 150ul The HBE1-Specific (66151-1-Ig) by Proteintech is a Monoclonal antibody targeting HBE1-Specific in WB, IHC, ELISA applications with reactivity to human samples 66151-1-Ig targets HBE1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: hemoglobin, epsilon 1 Observed Molecular Weight: 16 kDa GenBank Accession Number: NM_005330 Gene Symbol: HBE1 Gene ID (NCBI): 3046 RRID: AB_2881547 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P02100 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.