Proteintech, 66158-1-Ig, Fibrinogen Gamma Chain Monoclonal antibody

Size: 20ul / 150ul The Fibrinogen Gamma Chain (66158-1-Ig) by Proteintech is a Monoclonal antibody targeting Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66158-1-Ig targets Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human peripheral blood leukocyte, L02 cells, human peripheral blood platelets Positive IHC detected in: human liver cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: L02 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgM Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8817 Product name: Recombinant human FGG protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 90-437 aa of BC007044 Sequence: IQLTYNPDESSKPNMIDAATLKSRKMLEEIMKYEASILTHDSSIRYLQEIYNSNNQKIVNLKEKVAQLEAQCQEPCKDTVQIHDITGKDCQDIANKGAKQSGLYFIKPLKANQQFLVYCEIDGSGNGWTVFQKRLDGSVDFKKNWIQYKEGFGHLSPTGTTEFWLGNEKIHLISTQSAIPYALRVELEDWNGRTSTADYAMFKVGPEADKYRLTYAYFAGGDAGDAFDGFDFGDDPSDKFFTSHNGMQFSTWDNDNDKFEGNCAEQDGSGWWMNKCHAGHLNGVYYQGGTYSKASTPNGYDNGIIWATWKTRWYSMKKTTMKIIPFNRLTIGEGQQHHLGGAKQAGDV Predict reactive species Full Name: fibrinogen gamma chain Calculated Molecular Weight: 453aa,52 kDa; 437aa,50 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC007044 Gene Symbol: Fibrinogen Gamma Chain Gene ID (NCBI): 2266 RRID: AB_2881554 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph UNIPROT ID: P02679 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.