Proteintech, 66169-1-Ig, Caspase 9/P35 Monoclonal antibody

Size: 20ul / 150ul Caspase 9/P35 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA 66169-1-Ig targets Caspase 9/P35 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human lymphoma tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731]. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, sheep Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20813 Product name: Recombinant human Caspase 9 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 138-389 aa of BC002452 Sequence: DVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVAN Predict reactive species Full Name: caspase 9, apoptosis-related cysteine peptidase Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46 kDa, 35 kDa GenBank Accession Number: BC002452 Gene Symbol: Caspase 9 Gene ID (NCBI): 842 RRID: AB_2833257 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P55211 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.