Proteintech, 66173-1-Ig, CNPY2/MSAP Monoclonal antibody

Size: 20ul / 150ul The CNPY2/MSAP (66173-1-Ig) by Proteintech is a Monoclonal antibody targeting CNPY2/MSAP in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66173-1-Ig targets CNPY2/MSAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, rat liver tissue, human placenta tissue, pig liver tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2 Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6363 Product name: Recombinant human CNPY2,MSAP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-182 aa of BC065015 Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL Predict reactive species Full Name: canopy 2 homolog (zebrafish) Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 18-21 kDa GenBank Accession Number: BC065015 Gene Symbol: CNPY2 Gene ID (NCBI): 10330 RRID: AB_2881568 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y2B0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.