Proteintech, 66174-1-Ig, TPSAB1 Monoclonal antibody

Size: 20ul / 150ul The TPSAB1 (66174-1-Ig) by Proteintech is a Monoclonal antibody targeting TPSAB1 in WB, IHC, ELISA applications with reactivity to human samples 66174-1-Ig targets TPSAB1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human kidney tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP Predict reactive species Full Name: tryptase alpha/beta 1 Calculated Molecular Weight: 275 aa, 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC028059 Gene Symbol: TPSAB1 Gene ID (NCBI): 7177 RRID: AB_2881569 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15661 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.