Product Description
Size: 20ul / 150ul
The KLF15 (66185-1-Ig) by Proteintech is a Monoclonal antibody targeting KLF15 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples
66185-1-Ig targets KLF15 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: human skeletal muscle tissue
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).
Specification
Tested Reactivity: human, mouse, rat
Cited Reactivity: human, mouse
Host / Isotype: Mouse / IgM
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag4864 Product name: Recombinant human KLF15 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-416 aa of BC036733 Sequence: MVDHLLPVDENFSSPKCPVGYLGDRLVGRRAYHMLPSPVSEDDSDASSPCSCSSPDSQALCSCYGGGLGTESQDSILDFLLSQATLGSGGGSGSSIGASSGPVAWGPWRRAAAPVKGEHFCLPEFPLGDPDDVPRPFQPTLEEIEEFLEENMEPGVKEVPEGNSKDLDACSQLSAGPHKSHLHPGSSGRERCSPPPGGASAGGAQGPGGGPTPDGPIPVLLQIQPVPVKQESGTGPASPGQAPENVKVAQLLVNIQGQTFALVPQVVPSSNLNLPSKFVRIAPVPIAAKPVGSGPLGPGPAGLLMGQKFPKNPAAELIKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPFACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRSVRSVN Predict reactive species
Full Name: Kruppel-like factor 15
Calculated Molecular Weight: 416 aa, 44 kDa
Observed Molecular Weight: 44-55 kDa
GenBank Accession Number: BC036733
Gene Symbol: KLF15
Gene ID (NCBI): 28999
RRID: AB_2881580
Conjugate: Unconjugated
Form: Liquid
Purification Method: Thiophilic affinity chromatograph
UNIPROT ID: Q9UIH9
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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