Proteintech, 66193-1-Ig, GSNOR,ADH5 Monoclonal antibody

Size: 20ul / 150ul The GSNOR,ADH5 (66193-1-Ig) by Proteintech is a Monoclonal antibody targeting GSNOR,ADH5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66193-1-Ig targets GSNOR,ADH5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HuH-7 cells, human testis tissue, HepG2 cells, fetal human brain tissue, pig liver tissue, rat brain tissue, rat liver tissue Positive IHC detected in: mouse testis tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9511 Product name: Recombinant human GSNOR,ADH5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-374 aa of BC014665 Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI Predict reactive species Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide Calculated Molecular Weight: 374 aa, 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC014665 Gene Symbol: ADH5 Gene ID (NCBI): 128 RRID: AB_2881587 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P11766 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.