Proteintech, 66207-1-Ig, Beta-2-Microglobulin Monoclonal antibody

Size: 20ul / 150ul The Beta-2-Microglobulin (66207-1-Ig) by Proteintech is a Monoclonal antibody targeting Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications with reactivity to human samples 66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, Raji cells, LNCaP cells Positive IHC detected in: human prostate cancer tissue, human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human prostate cancer tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, monkey Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: beta-2-microglobulin Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 13 kDa GenBank Accession Number: BC032589 Gene Symbol: B2M Gene ID (NCBI): 567 ENSEMBL Gene ID: ENSG00000166710 RRID: AB_2881598 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P61769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.