Proteintech, 66213-1-Ig, USP5 Monoclonal antibody

Size: 20ul / 150ul The USP5 (66213-1-Ig) by Proteintech is a Monoclonal antibody targeting USP5 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66213-1-Ig targets USP5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, A549 cells, LNCaP cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7575 Product name: Recombinant human USP5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC005139 Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF Predict reactive species Full Name: ubiquitin specific peptidase 5 (isopeptidase T) Calculated Molecular Weight: 96 kDa Observed Molecular Weight: 95-105 kDa GenBank Accession Number: BC005139 Gene Symbol: USP5 Gene ID (NCBI): 8078 RRID: AB_2881604 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P45974 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.