Proteintech, 66219-1-Ig, N-cadherin Monoclonal antibody

Size: 20ul / 150ul The N-cadherin (66219-1-Ig) by Proteintech is a Monoclonal antibody targeting N-cadherin in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 66219-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: rabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells Positive IHC detected in: mouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:7500-1:30000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923). Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse, rat, pig, canine, monkey Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4996 Product name: Recombinant human N-cadherin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 33-350 aa of BC036470 Sequence: KTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ Predict reactive species Full Name: cadherin 2, type 1, N-cadherin (neuronal) Calculated Molecular Weight: 906 aa, 100 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC036470 Gene Symbol: N-cadherin Gene ID (NCBI): 1000 RRID: AB_2881610 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P19022 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.