Proteintech, 66223-1-Ig, ORAI1 Monoclonal antibody

Size: 20ul / 150ul The ORAI1 (66223-1-Ig) by Proteintech is a Monoclonal antibody targeting ORAI1 in WB, ELISA applications with reactivity to human, mouse, rat, pig samples 66223-1-Ig targets ORAI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:30000 Background Information Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, pig, monkey, bovine Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18050 Product name: Recombinant human ORAI1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-301 aa of BC015369 Sequence: MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA Predict reactive species Full Name: ORAI calcium release-activated calcium modulator 1 Calculated Molecular Weight: 301 aa, 33 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC015369 Gene Symbol: ORAI1 Gene ID (NCBI): 84876 RRID: AB_2881614 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96D31 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.