Proteintech, 66232-1-Ig, Pikachurin Monoclonal antibody

Size: 20ul / 150ul The Pikachurin (66232-1-Ig) by Proteintech is a Monoclonal antibody targeting Pikachurin in WB, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 66232-1-Ig targets Pikachurin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human retina tissue, PC-3 cells Positive IF-P detected in: rat eye tissue Positive IF/ICC detected in: Y79 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6362 Product name: Recombinant human EGFLAM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-152 aa of BC033177 Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK Predict reactive species Full Name: EGF-like, fibronectin type III and laminin G domains Calculated Molecular Weight: 111 kDa Observed Molecular Weight: 111 kDa, 55-60 kDa GenBank Accession Number: BC033177 Gene Symbol: Pikachurin Gene ID (NCBI): 133584 RRID: AB_2881623 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q63HQ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.