Proteintech, 66241-1-Ig, BrdU Monoclonal antibody

Size: 150ul The BrdU (66241-1-Ig) by Proteintech is a Monoclonal antibody targeting BrdU in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66241-1-Ig targets BrdU in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, L-929 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Background Information Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.Protocol for IHC:https://www.ptgcn.com/protocol/66241-1-IgIHC.pdfProtocol for IF:https://www.ptgcn.com/protocol/66241-1-IgIF.pdf Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Small molecule Predict reactive species Full Name: BrdU RRID: AB_2881630 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.