Proteintech, 66253-1-Ig, GNAS Monoclonal antibody

Size: 20ul / 150ul The GNAS (66253-1-Ig) by Proteintech is a Monoclonal antibody targeting GNAS in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, pig samples 66253-1-Ig targets GNAS in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: fetal human brain tissue, pig brain tissue Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa. Specification Tested Reactivity: human, pig Cited Reactivity: human, pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20530 Product name: Recombinant human NESP55,GNAS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 230-394 aa of BC002722 Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL Predict reactive species Full Name: GNAS complex locus Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC002722 Gene Symbol: GNAS Gene ID (NCBI): 2778 RRID: AB_2881641 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P63092 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.