Proteintech, 66254-1-Ig, PHOX2B Monoclonal antibody

Size: 20ul / 150ul The PHOX2B (66254-1-Ig) by Proteintech is a Monoclonal antibody targeting PHOX2B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 66254-1-Ig targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Neuro-2a cells Positive IHC detected in: neuroblastoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells, Neuro-2a cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13196 Product name: Recombinant human PHOX2B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 63-314 aa of BC017199 Sequence: GSCSLGTLRDHQSSPYAAVPYKLFTDHGGLNEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAAAAAAAAAKNGSSGKKSDSSRDDESKEAKSTDPDSTGGPGPNPNPTPSCGANGGGGGGPSPAGAPGAAGPGGPGGEPGKGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGWAPGPGPITSIPDSLGGPFASVLSSLQRPNGAKAALVKSSMF Predict reactive species Full Name: paired-like homeobox 2b Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 32-36 kDa GenBank Accession Number: BC017199 Gene Symbol: PHOX2B Gene ID (NCBI): 8929 RRID: AB_2881642 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99453 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.