Proteintech, 66271-1-Ig, CLPP Monoclonal antibody

Size: 20ul / 150ul The CLPP (66271-1-Ig) by Proteintech is a Monoclonal antibody targeting CLPP in WB, IHC, ELISA applications with reactivity to human, pig samples 66271-1-Ig targets CLPP in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: human heart tissue, pig heart tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid. Specification Tested Reactivity: human, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8420 Product name: Recombinant human CLPP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-277 aa of BC002956 Sequence: MWPGILVGGARVASCRYPALGPRLAAHFPAQRPPQRTLQNGLALQRCLHATATRALPLIPIVVEQTGRGERAYDIYSRLLRERIVCVMGPIDDSVASLVIAQLLFLQSESNKKPIHMYINSPGGVVTAGLAIYDTMQYILNPICTWCVGQAASMGSLLLAAGTPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPAST Predict reactive species Full Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli) Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 26 kDa, 30 kDa GenBank Accession Number: BC002956 Gene Symbol: CLPP Gene ID (NCBI): 8192 RRID: AB_2881656 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16740 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.