Proteintech, 66286-1-Ig, MYL3 Monoclonal antibody

Size: 20ul / 150ul The MYL3 (66286-1-Ig) by Proteintech is a Monoclonal antibody targeting MYL3 in WB, IHC, ELISA applications with reactivity to human, rat, mouse, pig samples 66286-1-Ig targets MYL3 in WB, IHC, RIP, ELISA applications and shows reactivity with human, rat, mouse, pig samples. Tested Applications Positive WB detected in: human skeletal muscle tissue, mouse heart tissue, human heart tissue, pig heart tissue, rat heart tissue Positive IHC detected in: human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905) Specification Tested Reactivity: human, rat, mouse, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24535 Product name: Recombinant human MYL3 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-195 aa of BC009790 Sequence: MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS Predict reactive species Full Name: myosin, light chain 3, alkali; ventricular, skeletal, slow Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22-25 kDa GenBank Accession Number: BC009790 Gene Symbol: MYL3 Gene ID (NCBI): 4634 RRID: AB_2881669 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P08590 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.