Proteintech, 66305-2-Ig, Vinculin Monoclonal antibody

Size: 150ul The Vinculin (66305-2-Ig) by Proteintech is a Monoclonal antibody targeting Vinculin in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, rabbit samples 66305-2-Ig targets Vinculin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: HGC-27 cells, HUVEC cells, LNCaP cells, MKN-45 cells, K-562 cells, human peripheral blood platelets, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific. Specification Tested Reactivity: human, mouse, rat, rabbit Cited Reactivity: rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24946 Product name: Recombinant human Vinculin protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 777-1066 aa of BC039174 Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ Predict reactive species Full Name: vinculin Calculated Molecular Weight: 1133 aa, 124 kDa Observed Molecular Weight: 124 kDa GenBank Accession Number: BC039174 Gene Symbol: Vinculin Gene ID (NCBI): 7414 RRID: AB_3670375 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P18206 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.